Extended Data Fig. 6: VIM, UBX, and PUB structural alignments and validation.
(a) Overlay of NTD-VIM from p97–UBXD1closed (colored) and gp78 (PDB 3TIW, white). (b) Map and model of the NTD and VIM from p97–UBXD1closed, colored as in (a). (c) Overlay of NTD-UBX from p97–UBXD1closed (colored) and UBXD7 (PDB 5X4L, white). (d) Map and model of the NTD and UBX from p97–UBXD1closed, colored as in (c). (e) Unsharpened, zoned map and model of UBX and PUB from p97–UBXD1-PUBout. (f) Unsharpened, zoned map and model of UBX and PUB from p97–UBXD1-PUBin. (g) Model of the UBX (yellow), PUB (gray), and UBX-PUB linker (light blue) from p97–UBXD1closed. (h) Unsharpened map of p97–UBXD1-PUBout.The VIM and helical lariat are colored the same as their corresponding protomers for clarity. (i) Unsharpened map of p97–UBXD1-PUBin. The VIM and helical lariat are colored the same as their corresponding protomers for clarity. (j) Filtered map of p97–UBXD1-PUBout, colored as in (d), showing weak density connecting the PUB and P5 CT tail. (k) As in (j), but for p97–UBXD1-PUBin.
