Extended Data Fig. 7: Validation of the helical lariat, UPCDC30245 binding, and additional structural features of p97–UBXD1H4 and p97–UBXD1open. | Nature Structural & Molecular Biology

Extended Data Fig. 7: Validation of the helical lariat, UPCDC30245 binding, and additional structural features of p97–UBXD1H4 and p97–UBXD1open.

From: The p97/VCP adaptor UBXD1 drives AAA+ remodeling and ring opening through multi-domain tethered interactions

Extended Data Fig. 7

(a) Sharpened map and model of Lα2-4, connecting strands of the helical lariat, and adjacent regions of P6 of p97–UBXD1meta, colored as in Fig. 1. (b) Overlay of P6 (blue) and Lα3-4 (orange) from p97–UBXD1closed with a p97 protomer (white) bound to UPCDC30245 (yellow) (PDB 5FTJ), aligned by the D2 domain (residues 483-763). (c) View of H4 and surrounding p97 density in p97–UBXD1H4 (left: unsharpened, right: sharpened) overlaid with the model for this state. (d) Sharpened map and model of the D2 domain of protomer P6 of p97–UBXD1H4, showing lack of density for α5′ (encircled). (e) Unsharpened map of protomer P1 of p97–UBXD1open. Density putatively corresponding to H4 is colored in green.

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