Fig. 5: Analysis of the helical lariat and VIM–H4 as conserved p97-remodeling motifs.
a, Domain schematics of UBXD1, ASPL and SVIP (not to scale). b,c, Overlay of the UBX–helical lariat of ASPL (residues 318–495 from PDB 5IFS, colored as in a) and UBXD1 (residues 270–441 from the p97–UBXD1closed model, in white) (b) and the VIM–‘H4-like’ region of SVIP (AlphaFold model, colored as in a) and UBXD1 (residues 50–93 from the AlphaFold model, in white) (c). d, Steady-state ATPase activity of p97 as a function of ASPL-C or SVIP concentration (normalized to activity at 0 nM adaptor). Data are from n = 3 independent experiments, each with three technical replicates. Data are presented as mean values from each independent experiment. Calculated IC50 values are also shown.
