Extended Data Fig. 5: THEMISY34F/Y34F mice show a significant developmental defect in CD4 thymocyte development.
From: THEMIS is a substrate and allosteric activator of SHP1, playing dual roles during T cell development
a-e, Mouse T cells (6 ~ 7-week old) were stained with anti-CD4−Qdot605, anti-CD8−APCCy7, anti-TCRb-PerCP-Cy5.5, and anti-CD69-PE-Cy7 in thymocytes or CD4-PerCP-Cy5.5 and CD8-FITC in splenocytes for 1 hour on ice. Quantification of the percentage of CD4SP (a), CD8SP (b), and mature cells (c) in total thymocytes or CD4+ (d) and CD8+ (e) T cells among total splenocytes isolated from male mice (left) and female mice (right) of the three genotypes (male: n = 16, 13, 10; female: n = 8, 6, 12).
