Extended Data Fig. 1: SSX1-H2AK119Ub nucleosome complex purification and 2D, 3D classification. | Nature Structural & Molecular Biology

Extended Data Fig. 1: SSX1-H2AK119Ub nucleosome complex purification and 2D, 3D classification.

From: Synovial sarcoma X breakpoint 1 protein uses a cryptic groove to selectively recognize H2AK119Ub nucleosomes

Extended Data Fig. 1

a. Schematic representation of the fusion oncoprotein SS18-SSX1 and GST-SSX1 construct. Arrows indicate the fusion site. b. Size exclusion chromatography (SEC) of SSX1-H2AK119Ub nucleosome complex. The peak fraction was analysed by SDS-PAGE and visualized with Coomassie blue-staining. c. SEC of SSX1-H2AK119Ub nucleosome complex after glutaraldehyde cross-linking. d. Selected 2D class averages generated from the particles used to reconstruct the SSX1-H2AK119Ub complex. e. The cryo-EM density map of SSX1-H2AK119Ub nucleosome at the level of 0.0045 in ChimeraX. The additional density of GST was visualized (as marked in red orange line) in d, e. f. SDS-PAGE and Native PAGE of SSX1-H2AK119Ub nucleosome complexes after glutaraldehyde cross-linking. These gels are representative of three independent experiments. The original gels are provided as source data.

Source data

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