Extended Data Fig. 3: Cryo-EM validation and example density for the structure of SSX1-H2AK119Ub nucleosome complex.
a. Top, the final 3.1 Å reconstruction of the SSX1-H2AK119Ub nucleosome complex (1:1 complex) contoured at a level of 0.015 is coloured according to local resolution with cyan and crimson representing the highest and lowest resolution, respectively. Bottom, euler angle distribution of all the particles used in the final reconstruction 3D reconstruction. b. Top, the final 3.6 Å reconstruction of the SSX1-H2AK119Ub nucleosome complex (2:1 complex) contoured at a level of 0.009 is coloured according to local resolution with cyan and crimson representing the highest and lowest resolution, respectively. Bottom, Euler angle distribution of all the particles used in the final reconstruction 3D reconstruction. c. FSC curve calculated between two independent half-maps from refinements in RELION reported at 0.143 FSC cutoff. d. Fourier shell correlation (FSC) curves between model and the Cryo-EM map. Resolution at FSC 0.5 is indicated. e. Representative regions of the cryo-EM density map for the H2A, H2B, H3, H4, SSX1, Ub, and DNA. Models are presented in sticks and densities in surfaces. f. Representative regions of the cryo-EM density map for the H2A/H2B acidic patch. g. Representative regions of the cryo-EM density map for H3-UbH2AK119 basic groove. h. Representative regions of the cryo-EM density map for H2A hydrophobic pocket.
