Extended Data Fig. 8: NtcA and NtcB independently bind to their respective boxes.
From: DNA looping mediates cooperative transcription activation
a, The sequences of 5′-Cy5 labeled dsDNA that are used in the EMSA assays. PnirA, 76-bp DNA containing wild-type NtcA and NtcB boxes; PnirA/ntcAm, a PnirA derivative bearing mutations at the conserved palindromic nucleotides of NtcA box; PnirA/ntcBm, a PnirA derivative bearing mutations at the conserved palindromic nucleotides of NtcB box. The mutated nucleotides are colored red. b, NtcA and NtcB bind to PnirA independently. NtcAWT, wide-type NtcA protein; NtcBWT, wide-type NtcB protein. c, Mutating the binding boxes of NtcA abolished NtcA binding activity but did not affect NtcB binding. d, Mutating the binding boxes of NtcB abolished NtcB binding activity but did not affect NtcA binding. All EMSA results are representatives of > 5 independent experiments that showed similar results. Source data are provided in the Source Data file.
