Extended Data Fig. 8: Small RNAs associated with AGO2 in resting and activated cells.
From: AGO2 silences mobile transposons in the nucleus of quiescent cells
(a) Representative western blot of fractions obtained following AGO2 pulldowns. Sup., supernatant; Inp., input; IP, immunoprecipitation. 1% of each fraction was loaded on the blot. Inputs from wild-type controls are shown as a control for the specificity of the antibody. (b) Composition of small RNA reads that map to annotated ncRNA loci in input (left) and IP samples (right) according to length distribution. Reads were normalized to total number of mapped reads. (c) Enrichment of miRNAs in AGO2 pulldowns in activated (Act.) and resting (Res.) primary B cells. (d) Number of reads mapping to miRNAs or TEs following pulldowns of AGO2 in resting B cells. The y-axis represents reads per million (RPM) of 20-24 nucleotide long RNAs. Data shown in C-D represents the average between two biological replicates.
