Extended Data Fig. 1: Ago2HA/HA mice are phenotypically normal and have intact Ago2 functions.
From: AGO2 silences mobile transposons in the nucleus of quiescent cells
(a) Targeting strategy. An 2xHA tag and short linker sequence were introduced directly downstream of start codon maintaining 5′UTR and promoter sequences intact. Position of the first exon (1) and length of homology arms are shown. (b) Western blot to MEFs obtained from heterozygous intercrosses. (c) Luciferase assay in MEFs showing intact repression of miRNA (orange) and AGO2 cleavage (red) reporters. Values represent mean of two biological replicates. Error bars represent standard deviation. p-values were calculated using a one-sided t-test. (d) Expected and observed numbers of genotypes obtained from heterozygous intercrosses at weaning. p-value was calculated with a Chi-square test. (e) Weigh of littermate animals at weaning (males: 14 wild-type, 25 heterozygous, 12 homozygous; females: 11 wild-type, 30 heterozygous, 9 homozygous). Data are presented as mean values +/− SD. p-values were calculated using a one-sided t-test. (f) Hematoxylin & Eosin-stained sections of tissues isolated from Ago2HA/HA animals and Ago2+/+ controls showing intact tissue morphology in homozygous animals.
