Table 1 Statistics for data collection and model refinement of cryo-EM determination
From: General transcription factor from Escherichia coli with a distinct mechanism of action
Data collection and processing | |
Magnification | 105,000 |
Voltage (kV) | 300 |
Detector | Gatan K3 |
Total images collected | 3,596 |
Electron exposure (e−/Å2) | 60 |
Defocus range (μm) | 1.7–2.5 |
Pixel size (Å) | 0.852 |
Symmetry imposed | C1 |
Initial particle images (no.) | 540,000 |
Final particle images (no.) | 177,841 |
Map resolution (Å) | 2.76 |
FSC threshold | 0.143 |
Refinement | |
Initial models used (PDB codes) | 6OUL, 2BN8 |
Model-to-map fit | 0.8454 |
R.m.s. deviations | |
Bond lengths (Å) | 0.013 |
Bond angles (°) | 1.449 |
Validation | |
MolProbity score | 1.89 |
Clashscore | 4.62 |
Poor rotamers (%) | 0.03 |
Ramachandran plot | |
Favored (%) | 95.13 |
Allowed (%) | 4.87 |
Disallowed (%) | 0.00 |
