Extended Data Fig. 7: p-Rab1AThr75 has a subtle effect on ER morphology in interphase cells.
a, Left, endogenous Rab1A-knockdown MDA-MB-231 cells co-expressing mCherry-tagged wild-type or mutant Rab1A and ER-GFP were localized by N-SIM. Scale bars = 5 μm. Right, quantification of ER three-way junctions in MDA-MB-231 cells with wild-type or mutant Rab1A overexpression. WT, n = 21; T75A, n = 21; T75D, n = 21. b, Endogenous Rab1A-knockdown MDA-MB-231 cells co-expressing mCherry-tagged wild-type or mutant Rab1A and ER-GFP were treated with nocodazole and analyzed by 3D-SIM. Left, representative images of high- or low-complexity ER. Red box regions were enlarged and subjected to 3D reconstruction. Scale bars = 5 μm. Right, percentage of cells co-expressing mCherry-tagged wild-type or mutant Rab1A and ER-GFP with high-complexity ER. WT, n = 286; T75A, n = 309; T75D, n = 229. Data from a, b were examined over three independent experiments. Data from a were analyzed using one-way Welch’s ANOVA test (P = 0.5245), and pairwise comparisons were performed using Dunnett’s T3 multiple comparisons test. Data are presented as mean ± s.e.m.
