Extended Data Fig. 9: Kinetic measurement of fluorescence anisotropy over a range of clamp loader concentrations.
From: Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM
The fluorescence anisotropy of clamp loader to fluorescently labeled primer-template DNA is measured over time and across clamp loader concentration. This complex was also bound to the non-hydrolysable analog ADP*BeF3. Very slow equilibration of the anisotropy readout is seen at low clamp loader concentration (between 0.009 uM and 0.039 uM). Clamp loader at a concentration of around 0.08 uM generates consistent anisotropy values after approximately 10,000 seconds (~2.5 hours).
