Extended Data Fig. 3: Electron density of wild-type and D110C T4 bacteriophage crystal structures.
From: Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM
a, The electron density map (2FoFc) of the re-refined wild-type clamp loader structure is shown centered around the active site of subunit D. The map is rendered at a contour level of 0.6176 e/Å3 (2.09 RMSD). Two view angles of the active site are shown. The nucleotide is the central axis of the view. b, The electron density map (2FoFc) of the D110C clamp loader mutant structure is shown centered around the active site of subunit D. The map is rendered at a contour level of 0.2253 e/Å3 (2.07 RMSD). Two view angles of the active site are shown. The nucleotide is the central axis of the view. C, The crystal structure of D110C mutated clamp loader (molecule A) is aligned against the wild-type clamp loader structure (gray). The alignment is performed on subunits C and D of both structures. The A, B, C, and E, subunits of the D110C structure are colored green, blue, magenta, yellow, and pink, respectively. The clamp subunits of the D110C structure are colored light blue, purple, and orange.
