Fig. 1: Mathematical modeling of deadenylation kinetics at single-nucleotide resolution.
From: Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution
a, Schematic of a dynamic model of deadenylation. b, In vitro deadenylation assay with human CCR4–NOT complex. c, Heatmap presentation of the deadenylation assay data. Position 1 represents the A20 RNA substrate, and position 21 represents the tailless 7-mer RNA. Column-specific unity-based normalization was applied for data visualization. d, Parameter estimation and deadenylation kinetics at each single-nucleotide position from the 3′ end. Error bars represent the standard error of parameter estimation. e, In silico deadenylation based on the estimated parameters. Column-specific unity-based normalization was applied for data visualization.
