Fig. 2: Stalling effect of a single guanosine residue on human CCR4–NOT complex.
From: Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution
a, Sequences of the synthetic 7-mer-A20 and 7-mer-A20G RNA substrates. Note the two guanosine residues at positions 7 and 14 for A20G. Position 21 represents the tailless 7-mer RNA. b, Schematic of the in vitro deadenylation analysis with human CCR4–NOT complex. c, In vitro deadenylation experiment with A20 and A20G RNA substrates (50 nM) and wild-type CCR4–NOT complex (25 nM). d, Left: heatmap analysis for A20 substrates. Column-specific unity-based normalization was applied for data visualization. Red arrowheads indicate the single-nucleotide positions 7, 14 and 21 from the 3′ end. Right: estimated deadenylation kinetics (nt min−1) based on the dynamic model. Error bars represent the standard error of parameter estimation. e, Deadenylation analysis as in d but with A20G RNA substrates. Error bars represent the standard error of parameter estimation. Heatmaps in d and e follow the same colour scale as in Fig. 1c,e.
