Fig. 3: Single pyrimidine residue produces a similar stalling effect but with greater impact at the terminal and antepenultimate positions.
From: Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution
a, Sequences of the synthetic 7-mer-A20U and 7-mer-A20C RNA substrates (50 nM) and wild-type CCR4–NOT complex (25 nM). Note the two pyrimidine residues at position 7 and 14 for A20U and A20C. Position 21 represents the tailless 7-mer RNA. b, Left: heatmap analysis for A20U substrates. Column-specific unity-based normalization was applied for data visualization. Red arrowheads indicate the single-nucleotide positions 7, 14 and 21 from the 3′ end. Right: estimated deadenylation kinetics (nt min−1) based on the dynamic model. Error bars represent the standard error of parameter estimation. c, Deadenylation analysis as in b but with A20C RNA substrates. Error bars represent the standard error of parameter estimation. Heatmaps in b and c follow the same colour scale as in Fig. 1c,e.
