Fig. 4: The distinct kinetic property of each catalytic subunit.
From: Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution
a–d, CCR4E240A:CAF1 mutant deadenylation analysis with A20 (a), A20G (b), A20U (c) and A20C (d) RNA substrates. e–h, CCR4:CAF1D40A mutant deadenylation analysis with A20 (e), A20G (f), A20U (g) and A20C (h) RNA substrates. Left: heatmap analysis. Column-specific unity-based normalization was applied for data visualization. Incubation was done for up to 48 min as in Fig. 3. Right: estimated deadenylation kinetics (nt min−1) based on the dynamic model. Error bars represent the standard error of parameter estimation. Heatmaps in a–h follow the same colour scale as in Fig. 1c,e.
