Fig. 5: Quantitative impact of mixed tails in terms of stalling deadenylation.

a, The relative slowdown (or time for single-nucleotide removal) for each nucleotide variant and nucleotide position from the first non-A residue (that is, position 7). Unity-based normalization was applied for each type of human CCR4–NOT complex experiment. A, adenosine; G, guanosine; U, uridine; C, cytidine. b, The equivalent number of additional adenosines in terms of the deadenylation activity of CCR4^E240A:CAF1 (n = 3) and CCR4:CAF1^D40A (n = 2). The impact of the first residue (that is, position 7) was estimated. The barplot represents the mean number of additional adenosines, and error bars represent the s.e.m. c, A quantitative model of CCR4–NOT-dependent deadenylation. The solid lines represent strong deadenylation activity, and dashed lines represent relatively weak activity. d, The equivalent number of additional adenosines in terms of the deadenylation activity of wild-type complexes (n = 4). Impacts of both residues (positions 7 and 14) were estimated and are presented separately. The barplot represents the mean number of additional adenosines, and error bars represent the s.e.m. e, The quantitative impact of mixed tails in terms of the number of additional adenosines.