Extended Data Fig. 2: Alternative conditions for in vitro deadenylation experiments.
From: Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution
a. Sequences of the synthetic 7-mer-A60 and 7-mer-A60G RNA substrates. b. Representative raw gel images from in vitro deadenylation experiment with A60 and A60G RNA substrates (50 nM) and wildtype CCR4-NOT complex (25 nM) of at least three technical and two biological replicates. c. Heatmap analysis for A60G substrates in (B). Column-specific unity-based normalization was applied for data visualization. Red arrowheads indicate the single-nucleotide positions 15, 30, and 45 from the 3′ end. d. Representative raw gel images after five-fold dilution from in vitro deadenylation experiment with A20 and A20G RNA substrates (250 nM) and wildtype CCR4-NOT complex (25 nM) of at least three technical and two biological replicates. e. Heatmap analysis for A20 and A20G substrates in (D). Column-specific unity-based normalization was applied for data visualization. Red arrowheads indicate the single-nucleotide positions 7, 14, and 21 from the 3′ end. f. Estimated deadenylation kinetics (nt / min) based on the dynamic model of (E). Error bars represent the standard error of parameter estimation.
