Extended Data Fig. 3: Supporting evidence related to Fig. 3.
From: Deadenylation kinetics of mixed poly(A) tails at single-nucleotide resolution
a. Representative raw gel images from in vitro deadenylation experiment with A20U and A20C RNA substrates (50 nM) and wildtype CCR4-NOT complex (25 nM) of at least three technical and two biological replicates. These images were used to quantitate deadenylation kinetics shown in Fig. 3. b. Re-analysis of TAIL-Seq data on HCMV-infected cells 10. The fraction of modified tails of HCMV RNA2.7 was calculated for RNAs with a poly(A) tail length of ≥25 nt (n = 1 TAIL-seq experiments).
