Extended Data Fig. 9: Details of the IL-12Rβ1D3-D5:Fab4 interaction.
From: Structures of complete extracellular receptor assemblies mediated by IL-12 and IL-23
a, Cartoon representation of the IL-12Rβ1D3-D5:Fab4 interaction. Receptor fragment in cartoon representation and Fab4 chain in surface representation. b, Detailed view of the IL-12Rβ1D3-D5:Fab4 interaction around residue R299 including some waters (red spheres) which are part of the interface. c, Dose-response curve of a SEAP based IL-23 reporter cellular assay in presence or absence of Fab4. EC50 values are reported together with their 95% confidence interval. 3 biological replicates, each in triplicate are shown. d, Cartoon representation of the crystal packing interaction of the better defined (lower B-factor) Fab4 copy interacting with two crystallographic copies (IL-12Rβ1 fragment and other complex not shown). e, The engineered Crystal Kappa mutation (magenta) near the C-terminus of the Light chain (wheat) forms an anti-parallel beta-sheet with the C-terminal beta strand of the Heavy chain (orange) of the neighboring Fab fragment allowing for a crystal lattice.
