Extended Data Fig. 3: CryoEM data processing workflow for the predimerized hIL-23:hIL-12Rβ1D1-D5-DAPK1302-330:hIL-23R-CaM complex.
From: Structures of complete extracellular receptor assemblies mediated by IL-12 and IL-23
Data processing was performed in CryoSPARC v3.3.228,29, and map post-processing was performed using DeepEMhancer25. Gold-standard Fourier Shell Correlation (FSC) curves are shown after applying either no mask (blue), a loose mask (green), or a tight mask (red) to both half maps before calculating the FSC. The corrected FSC (purple) is calculated using the tight mask with correction by noise substitution58. The estimated resolution at FSC = 0.143 (dotted purple lines) is shown for the corrected FSC curves (purple lines).
