Extended Data Fig. 8: Crosslinking mass-spectrometry analysis of the Pol II-TCR-ELOF1 complex. | Nature Structural & Molecular Biology

Extended Data Fig. 8: Crosslinking mass-spectrometry analysis of the Pol II-TCR-ELOF1 complex.

From: Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair

Extended Data Fig. 8

a. Crosslinking network between the subunits of the Pol II-TCR-ELOF1 complex. Crosslinks with a score above 3 that were detected at least twice are shown. b. Crosslinks from a mapped onto the Pol II-TCR-ELOF1 structure. Crosslinks within the permitted distance of 30 Å are shown in blue and crosslinks violating the distance are shown in red. Over 90% of crosslinks fall within the permitted distance. Crosslinks outside the permitted range likely emerge due to complex flexibility or technical errors. c. Histogram shows the number of crosslinks detected at a particular distance. d. Crosslinks used to identify the C-terminus of UVSSA. e. Crosslinks used to position the UVSSA hairpin in the Pol II pore.

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