Fig. 6: The C-terminus of UVSSA stimulates Pol II ubiquitylation and TFIIH recruitment.
a, Domain composition for UVSSA featuring novel elements. b, Zoom-in on the UVSSA C-terminus binding to downstream DNA. c, Quantification of transcription recovery (RRS) by 5-EU labeling after UV irradiation (3 h or 24 h; 12 J m−2) in RPE1-iCas9 UVSSA-KO cells complemented with the indicated GFP-tagged WT or mutant UVSSA. 5-EU levels are normalized to mock treatment for each cell line. The experiment was performed three times. Each colored circle represents one cell. Each black circle represents the mean of two technical replicates, with more than 80 cells collected per technical replicate. The black lines represent the mean of all three independent experiments. d, Endogenous Pol II-S2 immunoprecipitation (IP) after UV irradiation (9 J m−2, 1-h recovery) in RPE1-iCas9-WT or UVSSA-KO cells complemented with GFP-tagged WT or mutant UVSSA. TCR complex assembly and Pol II ubiquitylation were analyzed with the indicated antibodies. The data shown represent at least three independent experiments.
