Fig. 3: Spc24–Spc25 forms a rigid interface with MIS12C.
From: Structure of the human outer kinetochore KMN network complex
a, Cryo-EM density map of the MIS12C–Spc24–Spc25 interface. b, Molecular model of the MIS12C–Spc24–Spc25 interface, composed of rigidly docked Spc24–Spc25RWD formed from both a Dsn1 peptide contact with Spc24–Spc25 and Nsl1 peptide interface with Spc25; (c) and (d) refer to the views in c and d, respectively. c, Molecular details of the Dsn1 peptide interaction with Spc24–Spc25, highlighting a number of electrostatic interactions and the buried hydrophobic surface of Dsn1. d, Molecular details of the Nsl1 peptide interaction with Spc24–Spc25, similarly showing an electrostatic and geometric match between the Nsl1 peptide and Spc25 surface.
