Extended Data Fig. 9: HS-AFM fast imaging (50 frames per second) of an immobilized GltPh in extended lipid bilayers.
From: HS-AFM single-molecule structural biology uncovers basis of transporter wanderlust kinetics
HS-AFM frames of an isolated and immobilized GltPh trimer in extended lipid bilayers, viewed from the cytoplasmic side on the freshly cleaved mica with pretreatment of poly-Lys (Fig. 1, step 5b). The MEMPR method enables fast imaging of single immobilized molecules with unprecedented resolutions, 0.02 s/frame and 0.25 nm/pixel, and stability, for ~ 80 s in this example (Supplementary Video 13).
