Extended Data Fig. 1: GltPh PDB structures.
From: HS-AFM single-molecule structural biology uncovers basis of transporter wanderlust kinetics
(a), (b) and (c) PDB structures of GltPh outward-facing state open (OFS open, light blue, PDB 6X17), outward-facing state closed (OFS closed, dark blue, PDB 4OYE), inward-facing state open (IFS open, yellow, PDB 6X12), and inward-facing state closed (IFS closed, green, PDB 4P19) states: (a) Transport domain (blue) and trimerization domain (gray). (b) Superimposed open and closed structures of the OFS (top) and IFS (bottom) GltPh. Large domain movements present in the IFS structures. Inset: positions of the hairpin 2 (HP2), showing the open and closed ligand binding pocket. (c) Surface representations of the structures from the extracellular side (top), in a side view (middle), and from the cytoplasmic side (bottom). Black arrowheads: Trimerization domain height levels on the cytoplasmic side. Red arrowheads: Transport domain height levels on the cytoplasmic side. The height difference estimates indicate the height protrusion of the transport domain relative to the trimerization domain, viewed from the cytoplasmic side.
