Extended Data Fig. 1: Purification and characterization of AC5.
From: Structure of adenylyl cyclase 5 in complex with Gβγ offers insights into ADCY5-related dyskinesia
(A) AC5 was purified using anti-FLAG M2 affinity resin and analyzed by Coomassie blue stained SDS-PAGE. The AC activity of purified AC5 was determined in the presence of 10 mM MgCl2, 0.5 mM ATP at the indicated concentrations of (B) forskolin or (C) Gαs·GTPγS. The data shown are the mean ± SD from three independent assays.
