Extended Data Fig. 6: Integrator depletion alters the total RNAP II occupancy at actively transcribed genes.
From: Transcription directionality is licensed by Integrator at active human promoters
a. IGV tracks of RNU11 and RNU12 loci from total RNAPII ChIP-seq results after INTS11 depletion. b. IGV tracks of EP300 and EZH2 loci from RNAPII ChIP-seq results upon INTS11 depletion. c. Boxplots of 2121 highly expressed gene RNAPII ChIP-seq signals in PROMPTs (top) and gene bodies (bottom). P values were calculated by two-sided Mann-Whitney test. See Extended Data Fig. 1 for description of boxplots. d. Western blot of TAF1 and TAF3 protein levels after INTS1 and INTS11 depletion in whole cells and chromatin, for INTS1-AID2 cell line “-” represents equal-volume DMSO treatment as a control, and “+” represents 10 µM 5-Ph-IAA treatment, representing from three independent experiments with similar results. e. Heatmap of TAF1 ChIP-seq in a 2 kb window around TSS sites of 10428 genes and 2121 active genes. f. IGV tracks of KLF2 and NR4A1 loci from RNAP II ChIP-seq results upon EGF induction and INTS11 depletion. g. Box plots of RNAPII ChIP-seq signals at the 1026 EGF-induced gene loci (PROMPTs, left; gene bodies, right). P values were calculated by two-sided Mann-Whitney test. See Extended Data Fig. 1 for description of boxplots.
