Extended Data Fig. 7: scDyad&T-seq enables combined measurement of DNA methylation levels, 5mCpG maintenance levels and the transcriptome from the same cell.
(a) Genome-wide methylation and 5mCpG maintenance levels of single K562 cells treated with (+) or without (–) 0.6 µM Decitabine for 24 hours. (b) 5mCpHpG maintenance levels of single K562 cells treated with (+) or without (–) 0.6 µM Decitabine for 24 hours. Data is based on 134 cells treated with Decitabine and 143 cells not treated with Decitabine. (c) Number of genes detected as a function of transcriptome-derived reads sequenced per cell in scDyad&T-seq. Data corresponds to SL grown single E14 cells. (d) Coverage of CpG sites as a function of genomic DNA-derived reads sequenced per cell in scDyad&T-seq. Data corresponds to SL grown single E14 cells. (e,f) Coverage of individual CpG sites in scDyad&T-seq. Data corresponds to SL grown single E14 cells. (g,h) Coverage of individual CpG sites in M-M-Dyad-seq. Data corresponds to SL grown E14 mESCs. (i) Coverage of CpG sites and the number of genes detected per cell in scDyad&T-seq (blue) and scM&T-seq (red)45. The diameter of each circle corresponds to the read depth at which a cell was sequenced. Data in this panel corresponds to SL cultured E14 mESCs. (j) Distribution of CpG sites that are detected by different methods (scDyad&T-seq, scMspJI-seq and scBS-seq) over genomic regions of varying GpC density.
