Fig. 1: Design of DHFR RNCs.
From: Resolving chaperone-assisted protein folding on the ribosome at the peptide level
a, Structure of E. coli DHFR (PDB 5CCC). b, Schematic illustrations of stall-inducing constructs. The stall site is indicated in red, and subdomains are colored gold (discontinuous loop subdomain, DLD) or bronze (adenosine binding subdomain, ABD), with the artificial linker in FL + 58RNC in black. The region of the NC expected to span the exit tunnel is indicated. PTC, peptidyl transfer center. c, Secondary structure elements in DHFR, colored according to subdomains as in b. Sites where the stall sequence was inserted are marked with arrows.
