Fig. 2: HDX MS of RNCs.
From: Resolving chaperone-assisted protein folding on the ribosome at the peptide level
a, Schematic of HDX MS experiment. Purified RNCs are deuterated for 10 s, 100 s or 1,000 s before the reaction is quenched and the entire system is digested into peptides. Peptides are resolved in solution (LC, liquid chromatography) and in the gas phase (IMS, ion-mobility spectrometry), followed by analysis of deuterium incorporation by MS. b, Relative deuterium uptake of DHFR peptides after 10 s exposure to deuterium as a percentage of the maximum possible exchange, for isolated DHFR (FL DHFR) and FL + 58RNC. A maximally deuterated control sample (FL DHFR maxD) is shown as a reference. Lines are a guide for the eye only. Data are presented as mean values of two to four replicates. Error bars, s.d.; n = 3 or n = 4 independent experiments. See also Extended Data Fig. 3 and Supplementary Data 1.
