Table 1 Kinetic parameters of GluAx constructs
| Â | Peak (pA) | Ï„w, desensitization (ms) | Ï„recovery (ms) | Steady state | ||||
|---|---|---|---|---|---|---|---|---|
pH 7.4 | pH 5.5 | pH 7.4 | pH 5.5 | pH 7.4 | pH 5.5 | pH 7.4 | pH 5.5 | |
A2 wt | 468 ± 371 (27) | 299 ± 244 (27) | 14.3 ± 2.3 (27) | 3.7 ± 1.2 (27) | 15.6 ± 2.9 (22) | 28.8 ± 3.6 (22) | 8.6 ± 2.8 (27) | 1.6 ± 1.1 (27) |
A2delNTD (n = 7) | 546 ± 407 | 378 ± 320 | 18.9 ± 1.2 | 4.9 ± 0.3 | 17.7 ± 2.2 | 21.3 ± 1.5 | 11.9 ± 2.3 | 3.7 ± 2.0 |
A2NTD_A1 (n = 8) | 846 ± 303 | 521 ± 228 | 13.3 ± 1.5 | 4.1 ± 0.7 | 27.0 ± 2.5 | 33.9 ± 3.3 | 8.6 ± 1.5 | 3.3 ± 1.1 |
A2H208M (n = 6) | 331 ± 49 | 200 ± 39 | 10.6 ± 2.0 | 2.9 ± 0.7 | 29.1 ± 1.9 | 37.0 ± 3.1 | 6.1 ± 2.2 | 2.4 ± 1.4 |
A2F231A (n = 9) | 254 ± 108 | 155 ± 73 | 11.1 ± 1.3 | 3.0 ± 0.4 | 27.2 ± 2.9 | 36.3 ± 4.6 | 7.8 ± 2.0 | 3.0 ± 1.5 |
A1wt (n = 7) | 329 ± 159 | 228 ± 129 | 5.1 ± 0.8 | 3.0 ± 0.7 | 138 ± 14 | 202 ± 36 | 3.8 ± 1.4 | 1.9 ± 0.9 |
A1NTD_A2 (n = 7) | 315 ± 126 | 171 ± 53 | 4.6 ± 1.0 | 2.1 ± 0.2 | 116 ± 16 | 284 ± 46 | 1.3 ± 0.8 | 0.4 ± 0.3 |
