Fig. 2: Pol ε Fingers adopt open, ajar and closed conformations. | Nature Structural & Molecular Biology

Fig. 2: Pol ε Fingers adopt open, ajar and closed conformations.

From: Structural basis for processive daughter-strand synthesis and proofreading by the human leading-strand DNA polymerase Pol ε

Fig. 2

a, Ribbon models of Pol ε cat in complex with PCNA, substrate DNA and an incoming nucleotide in open, ajar (semiclosed) and closed conformations. b, Superposition of the Pol ε cat ternary complex in the three different states shown in a. The two α-helices of the Finger domain are indicated for the open (teal), ajar (gray) and closed (orange) conformations. The rotation symbol in this figure indicates the view relative to a. c, Coordination of the incoming nucleotide within the active site in the respective states. Side chains of the indicated amino acid positions are shown as sticks. Dashed lines indicate electrostatic and hydrogen-bond interactions between the Pol ε Fingers and the triphosphate moiety of the incoming nucleotide. d, Cryo-EM density (shown as mesh) for the incoming nucleotide and the coordinating peptide backbone at the surface of the Palm domain.

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