Fig. 3: Cryo-EM structures of Pol ε at a T–C-mismatched DNA substrate. | Nature Structural & Molecular Biology

Fig. 3: Cryo-EM structures of Pol ε at a T–C-mismatched DNA substrate.

From: Structural basis for processive daughter-strand synthesis and proofreading by the human leading-strand DNA polymerase Pol ε

Fig. 3

a, DNA oligonucleotide pair that was used as the substrate for reconstitution. b–f, The four distinct conformational states that were observed for Pol ε at mismatched DNA. Shown are the cryo-EM density maps (b) and corresponding atomic models underneath (c–f). The locations of active sites for polymerization (pol) and proofreading (exo) are indicated in the complete ribbon models; the mismatched nucleotide is colored in red (c). A top view (rotation symbol relative to c, showing only indicated components) illustrates the Thumb domain movement, rearrangements in the Anchor loop and the handover of the DNA substrate between the Thumb and P domains (d). Close-up views show the change in the interfaces between the DNA substrate and the P (salmon) and Thumb (blue) domains (e) and between the DNA substrate and the Anchor loop (green) (f). The DNA and coordinating side-chain residues of Pol ε are shown as sticks; the mismatched nucleotide is colored red.

Back to article page