Fig. 5: Retrieval of erroneous DNA from the pol active site. | Nature Structural & Molecular Biology

Fig. 5: Retrieval of erroneous DNA from the pol active site.

From: Structural basis for processive daughter-strand synthesis and proofreading by the human leading-strand DNA polymerase Pol ε

Fig. 5

a, Superposition of Pol ε cat in the Post-Insertion (gray) and Pol Arrest (colorized) states. Dashed arrows indicate the outward opening movement of the Thumb domain. b, View of the pol active site comparing the Post-Insertion (left) and the Arrest (right) states. The incoming nucleotide is absent in the structure of the Arrest state but is depicted in both panels for reference. The rotation symbol indicates the view relative to a, left. c, Path of the DNA double helix in the two states shown as a cartoon representation of the atomic model and as spheres at the centroids between the backbone phosphates of the paired strands. The Thumb domain is shown as a simplified cartoon and its twisting movement is indicated by the rotation symbol. The DNA distortion in positions n-2 to n-5 (with n-1 being the terminal mismatch) in the Arrest state is indicated and becomes visible from overlapping spheres and the kinked trace of the centroids. The upstream portion of the DNA tilts away from the Thumb and toward the P domain.

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