Extended Data Fig. 7: Additional data on the interplay between MeCP2 and its binding partners.
From: Differential dynamics specify MeCP2 function at nucleosomes and methylated DNA
a, Representative kymographs of AF488-nucleosome-containing unmethylated DNA tethers bound with Cy5-MeCP2 and Cy3-H1. Arrows denote nucleosome positions. b, Representative kymograph of an unmethylated bare DNA tether bound with LD655-MeCP2 and Cy3-H1 (4 nM each). c, Mass distribution for mononucleosomes containing 207-bp DNA (blue) and for those incubated with Cy3-MeCP2 and H1 (yellow). Peak mass values are indicated. d, Representative kymograph of a nucleosome-containing unmethylated DNA tether bound with Cy5-MeCP2 and Cy3-H1 while being pulled to high forces. e, Representative kymograph of a bare methylated DNA tether incubated with LD655-TBLR1 showing a lack of binding. f, Representative kymograph of a bare unmethylated DNA tether incubated with LD655-TBLR1 and Cy3-MeCP2.
