Fig. 4: Nucleosome targeting by MeCP2 requires domains outside its MBD.
From: Differential dynamics specify MeCP2 function at nucleosomes and methylated DNA
a, Domain structure of full-length (FL) MeCP2 and three truncations R270X, K210X, and R162X. b, Fraction of nucleosomes loaded on unmethylated DNA tethers that were colocalized with MeCP2 after incubation with 2 nM FL (n = 9 independent tethers), R270X (n = 11 independent tethers), K210X (n = 17 independent tethers), R162X (n = 8 independent tethers), T158M (n = 4 independent tethers) or P225R (n = 7 independent tethers) Cy3-MeCP2. Each dot represents data from one independent tether. The bars represent mean and s.d. Significance was calculated using a one-way ANOVA with Tukey’s test for multiple comparisons. c–f, Representative kymographs of an LD655-nucleosome-containing unmethylated DNA tether bound with Cy3-MeCP2 (c), Cy3-MeCP2R270X (d), Cy3-MeCP2K210X (e) or Cy3-MeCP2R162X (f). The arrowheads denote nucleosome positions.
