Extended Data Fig. 6: High-resolution cryo-ET structure of HTLV-1 MA126CANC tubes.
From: Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice
a) FSC for the CA-NTD from MA126CANC tubes, showing a resolution of 3.4 Å at the 0.143 criterion. b) Local resolution measurements for the cryo-EM density map of the CA layer of HTLV-1 MA126CANC tubes tubes. The CA-NTD and CA-CTD are annotated in the side view, highlighting again the weak density for the CA-CTD. The CA hexamer center is indicated with a small black hexagon. c) Model of HTLV-1 CA-NTD (cyan) refined into the 3.4 Å cryo-EM density map. The CA hexamer center is indicated with a small black hexagon. Below, representative EM densities for two helices of the CA-NTD are shown with the respective fit. At this resolution, the helical pitch and densities for larger side chains are visible. d) Low-pass-filtered isosurface representation of the subtomogram average of the C2-symmetric CA hexamer from HTLV-1 MA126CANC tubes, as seen in a side view (left) or from within the tube, showing the CA-CTD. This panel relates to the Fig. 2d, which shows the same map at the unfiltered 3.4 Å resolution. The orange dashed rectangle indicates the CA-CTD layer, which is not visible at high resolution in Fig. 2d. e) Distances between adjacent CA-NTD trimers and CA-CTD dimers.
