Extended Data Fig. 4: Expression and purification of retrovirus CA proteins for in vitro experiments.
From: Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice
a) Samples of purified HTLV-1 CA protein variants were separated by SDS–PAGE (15%) and stained via Coomassie brilliant blue. Positions of molecular mass standards (in kiloDaltons, kDa) are indicated. HTLV-1 CA is full-length from residues 131–344 (using Gag-numbering), CA-NTD includes residues 131–258 and CA-CTD includes residues 259–344. b) Negative Stain TEM micrographs of HTLV-1 CA tubes. Scale bars are annotated in the figure. c) Samples of purified HIV-1 CA protein variants were separated by SDS–PAGE (15%) and stained via Coomassie brilliant blue. Positions of molecular mass standards (kDa) are indicated. HIV-1 CA is full-length from residues 133–363 (using Gag-numbering), CA-NTD includes residues 133–278 and CA-CTD includes residues 279–363. d) Negative Stain TEM micrographs of HIV-1 CA assemblies. Scale bars are annotated in the figure. The shown gels and negative stain micrographs are representative for at least 3 repetitions of the same experiment which yielded similar results.
