Extended Data Fig. 5: Image processing and classification of HTLV-1 MA126CANC tubes.
From: Distinct stabilization of the human T cell leukemia virus type 1 immature Gag lattice
a) Image processing workflow for the subtomogram averaging and multiparticle refinement of the CA-NTD layer from HTLV-1 MA126CANC tubes. Please see Materials and Methods for more details. b) Classification of tubes based on geometry. Different tubes have different geometries, with varying helical rise and pitch. For improved structure determination, tubes were grouped into classes according to their hexamer orientation (blue arrow) with respect to the tubes axis (black arrow). c) Histogram of abundance of different tube groups. d) Refined EM-density maps for the individual tube groups. Upper left corner – angle group; lower left corner – number of particles; lower right corner – resolution.
