Table 1 Cryo-EM data collection, refinement and validation statistics
Data collection and processing | |
Magnification | 165,000 |
Voltage (kV) | 300 |
Electron exposure (e− per Å2) | 48.1 |
Defocus range (μm) | −1.5 to −3.5 |
Pixel size (Å) | 0.82 |
Symmetry imposed | C1 |
Initial particle images (no.) | 1,069,057 |
Final particle images (no.) | 121,194 |
Map resolution (Å) | 3.6 |
FSC threshold | 0.143 |
Map resolution range (Å) | 10.6-2.0 |
Refinement | |
Initial model used (PDB code) | |
Model resolution (Å) | 3.58 (masked) |
FSC threshold | 0.5 |
Map sharpening B factor (Å2) | 67.0 |
Model composition | |
Nonhydrogen atoms | 10,132 |
Protein residues | 1,374 |
Ligands | 1 |
B factors (Å2) | |
Protein | 52.95 |
Ligand | 51.30 |
Root-mean-square deviations | |
Bond lengths (Å) | 0.024 |
Bond angles (°) | 3.170 |
Validation | |
MolProbity score | 2.27 |
Clashscore | 0 |
Poor rotamers (%) | 3% |
Ramachandran plot | |
Favored (%) | 92% |
Allowed (%) | 8% |
Disallowed (%) | 0% |
