Extended Data Fig. 6: Stilbene conjugation site in the binding pocket formed by the A and C subunits determined by crystallography and cryo-EM.
From: The conformational landscape of human transthyretin revealed by cryo-EM
The top panels show electron density below noise level from a 1.5 Å resolution crystal structure. The stilbene moiety seats at a crystallographic symmetry axis, resulting in two-fold averaging of the density. The bottom panels show the same region in our cryo-EM canonical state reconstruction, which was generated without imposing symmetry, showing the asymmetric binding pose of the ligand.
