Table 1 Cryo-EM data collection, refinement and validation statistics
From: Local structural flexibility drives oligomorphism in computationally designed protein assemblies
KWOCA 18 | KWOCA 70 | I32-10 | |
|---|---|---|---|
Data collection and processing | |||
Magnification | 36,000 | 36,’000 | 36,000 |
Voltage (kV) | 200 | 200 | 200 |
Electron exposure (e−/Å2) | 50.1 | 50.1 | 60.0 |
Defocus range (μm) | −(1.0–1.5) | −(1.0–1.5) | −(0.8–1.8) |
Pixel size (Å) | 1.15 | 1.15 | 1.16 |
Symmetry imposed | D2 and D5 | D2 and D3 | C1 |
Initial particle images (no.) | 300,970 | 360,972 | Variable |
Final particle images (no.) | 45,922 and 22,981 | 32,790 and 302,683 | Variable |
Map resolution (Å) | 8.0 and 7.1 | 7.2 and 7.1 | ~10 to 25 |
FSC threshold | |||
Map resolution range (Å) | NA | NA | NA |
