Extended Data Fig. 6: Cryo-EM structure determination of the AP5βtrunk-SPG11-SPG15 complex.
From: Structural basis for membrane remodeling by the AP5–SPG11–SPG15 complex
a, Representative motion-corrected cryo-EM micrograph (of 5,644 micrographs) of the AP5βtrunk-SPG11-SPG15 complex. b, Representative 2D class averages for the AP5βtrunk-SPG11-SPG15 complex. c, Flow chart of cryo-EM data processing. NU-refinement: nonuniform refinement. d, Angular particle distribution calculated in cryoSPARC for particle projections. The heatmap shows the number of particles for each viewing angle. e, The FSC plots are between two independently refined half-maps with no mask (blue), spherical mask (orange), loose mask (green), tight mask (red), and corrected (purple). A cut-off of 0.143 (blue line) was used to estimate the resolution. f, AP5βtrunk-SPG11-SPG15 map color-coded by the local resolution estimation.
