Extended Data Fig. 7: Apo cryo-EM data collection and initial processing.
From: Human polymerase θ helicase positions DNA microhomologies for double-strand break repair
Data collection and initial processing described for apo PolθH. CTF correction was performed in cryoSPARC live, and micrographs were exported to cryoSPARC for particle picking, extraction, and further processing based on particle presence in 2D and map homogeneity in 3D as described in the methods. One representative micrograph is shown with picked particles in red. For 2D sorting jobs, representative classes are shown. Initial models were created Ab initio from particles.
