Extended Data Fig. 6: Processing workflow for the cryo-EM data set of proteasomes incubated with NUB1/FAT10 for 30 s.
From: NUB1 traps unfolded FAT10 for ubiquitin-independent degradation by the 26S proteasome
Preincubated FAT10-Eos (5 µM) and NUB1 (6 µM) were added to hs26S proteasome (2 µM) for 30 s before plunge freezing for Cryo-EM. The further classification of models for the non-processing proteasome is described in Figures E7 and S10. CryoSparc was used for all data processing and ChimeraX for visualization.
