Extended Data Fig. 8: Cryo-EM analysis of the REPAIR complex.
From: Structural insights into RNA-guided RNA editing by the Cas13b–ADAR2 complex
(a) Domain structure of human ADAR2. dsRBD, dsRNA-binding domain. (b) Nucleotide sequence of the modified dsRNA used for the crystallographic analysis25. (c) Crystal structure of the ADAR2DD–dsRNA complex (PDB: 5ED1)25. Inositol hexaphosphate (IHP) is bound to the enzyme core and stabilizes the protein fold. The 8-azanebularine (8-AZ) is flipped out from the RNA duplex and recognized by the ADAR2DD active site. (d) Single-particle cryo-EM image processing workflow. (e) Representative micrograph at a magnification of ×105,000. (f) Representative 2D averaged class images. (g) FSC curves. The map-to-map FSC curve was calculated between the two independently refined half-maps after masking (blue line), and the overall resolution was determined by the gold standard FSC = 0.143 criterion. The map-to-model FSC was calculated between the refined atomic models and maps (red line). (h) Directional FSC plots calculated by the 3DFSC server. (i) Angular distribution of the particles in the final reconstruction. (j) Cryo-EM density maps, colored according to the local resolution. (k) Fitting of the ADAR2 domain to the cryo-EM density map. A close-up view of the density map around 8-AZ and IHP is shown on the right.
