Extended Data Fig. 2: Cryo-EM analysis of the PspCas13b–crRNA binary complex.
From: Structural insights into RNA-guided RNA editing by the Cas13b–ADAR2 complex
(a) Single-particle cryo-EM image processing workflow. (b) Representative micrograph at a magnification of ×105,000. (c) Representative 2D averaged class images. (d) FSC curves. The map-to-map FSC curve was calculated between the two independently refined half-maps after masking (blue line), and the overall resolution was determined by the gold standard FSC = 0.143 criterion. The map-to-model FSC curve was calculated between the refined atomic models and maps (red line). (e) Directional FSC plots calculated by the 3DFSC server. (f) Angular distribution of particles in the final reconstruction. (g and h) Cryo-EM density maps, colored according to the local resolution (g) and the structural domains (h).
