Extended Data Fig. 1: Cryo-EM structure determination workflow.
From: Structure, assembly and inhibition of the Toxoplasma gondii respiratory chain supercomplex
(a) Representative micrograph with T. gondii and C. sabaeus supercomplex particles indicated in blue and orange, respectively. (b) Representative 2D classes of the T. gondii supercomplex. (c) Representative 2D classes of C. sabaeus supercomplex. (d) Data processing scheme, highlighting the reference-based 3D classification to separate T. gondii and C. sabaeus ATP synthase (purple, light green), mitochondrial large subunit (yellow) and putative mitochondrial chaperonin (teal) from T. gondii and C. sabaeus supercomplexes (blue and orange). (e) Fourier Shell Correlation (FSC) curves. (f) Representative micrograph with T. gondii complex III from IP. (g) Representative 2D classes of the T. gondii complex III. (h) Data processing scheme for complex III.
